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Laboratoire de Biopharmacie / Biopharmaceutical laboratory

Membre / part of EA 4678 CIDAM

Conception, Ingénierie et Développement de l'Aliment-Médicament

Contact :

Directeur / Head : Prof. Dr. J-M CARDOT

Laboratoire de Biopharmacie, Faculté de Pharmacie,
28 Place Henri Dunant , B.P. 38,
F-630001 Clermont-Ferrand Cedex 01  FRANCE
Tél. /Phone : +33 (0)4 73 17 79 68
Télécopie / Fax : +33 (0)4 73 17 79 59
E-Mail : j-michel.cardot@udamail.fr

Mots clés / Key words :

Dissolution et évaluation in vitro, biodisponibilité et pharmacocinétique, corrélations in vitro/in vivo.
Dissolution and in vitro evaluation, bioavailability and pharmacokinetics, in vitro/in vivo correlations.

Effectifs / Members :

Enseignants-chercheurs / Researchers :

Prof. Dr. Eric Beyssac, e.mail : eric.beyssac@udamail.fr
Prof. Dr. Jean-Michel Cardot, e.mail : j-michel.cardot@udamail.fr
Dr. Ghislain Garrait, e.mail : gislain.garrait@udamail.fr
Dr. Valérie Hoffart, e.mail : valerie.hoffart@udamail.fr
Dr. Pascale Gauthier (Associée) e.mail : pascale.gauthier@udamail.fr

 

Thésards. Ph. D. students : 2

Matériel / Equipement :

Evaluation in vitro : Panier et palettes (USP I and II Paddles and Basket) : AT7, AT6 ; Cellule à flux continu (USP IV Flow through cells) : CE6 CE7; Biodis (USP III); Cellule de Frantz (Frantz Cells) ; Paddle over disk (Paddle over disk), Impacteurs à cascade (Cascate Impactors) ; Systèmes digestifs artificiels (Artifical digestive systems) : TIM, MYS.
Galénique (Formulation)  :  formes sèches, formes enrobées, formes pressurisées, formes semi solides (tablets, coating, aerosol, gels, emulsion, microsphere, etc.)
Analytique : HPLC-UV, HPLC-DAD, GC, PSD, FTIR
Evaluation de données (softwares) : Kinetika, Phoenix (Winnonlin), Mathlab, SAS

Publications internationales 2005-2015 :

Benichou Ac, Armanet M, Bussière A, Chevreau N, Cardot Jm, Tétard J. AProprietary blend of quail egg for the attenuation of nasal provocation with a standardized allergenic challenge: a randomized, double-blind, placebo-controlled study. Food Sci Nutr. 2014 Nov;2(6):655-63.

Beyssac E And Lavigne J. Dissolution Study Of Active Pharmaceutical Ingredients Using The Flow Through Apparatus Usp 4. Dissolution Technologies. 23 - 25 May 2005.

Blanquet S, Garrait G, Beyssac E, Perrier C, Denis S, Hébrard G, Alric M. Effects Of Cryoprotectants On The Viability And Activity Of Freeze Dried Recombinant Yeasts As Novel Drug Delivery Systems Assessed By An Artificial Digestive System. Eur. J. Pharm. Biopharm. 61 : 32-39, 2005.

Blanquet-Diot S, Denis S, Chalancon S, Chaira F, Cardot Jm, Alric M., Use Of Artificial Digestive Systems To Investigate The Biopharmaceutical Factors Influencing The Survival Of Probiotic Yeast During Gastrointestinal Transit In Humans.,Pharm Res. 2011 Nov 9.

Bohbot J-M, Cardot J-M. Vaginal Impact Of The Oral Administration Of Total Freeze-Dried Culture Of Lcr 35 In Healthy Women, Infect Dis Obstet Gynecol. ;2012:503648. Doi: 10.1155/2012/503648.

Cardot JM, Beyssac E, Alric M. In Vitro- In Vivo Correlation Importance Of Dissolution In IVIVC. Dissolution Technology, 2007, 14(1) : 15-19.

Cardot JM, Beyssac E. Control Of Cleaning. Bioforum Europe 2007, Vol. 11, No. 5: Pp 36-38.

Cardot JM, Beyssac E. The Basics Of Cleaning And Cleaning Validation. Bioprocess International, 2007, Vol. 5, No. 6: Pp 66-76.

Cardot J-M, In Vitro- In Vivo Correlation, Indian Journal Of Pharmacology, 2009, 40, 1

Cardot J-M., Beyssac E, Control Of Cleaning, Bioforum Europe 2007, Vol. 11, No. 5:36-38

Cardot J-M., Beyssac E, The Basics Of Cleaning And Cleaning Validation, Bioprocess International 2007, Vol. 5, No. 6:66-76

Cardot J-M., Beyssac E. And Alric M., In Vitro- In Vivo Correlation Importance Of Dissolution In Ivivc, Dissolution Technology, 2007, 14(1), 15-19

Cardot J-M., Davit B., In Vitro- In Vivo Correlations: Tricks And Traps, Aaps Journal 2012, Sep;14(3):491-9

Cardot J-M., Washing Or Throwing? Cleaning Vs. Dedicated Or Disposable Equipment, Bioforum Europe 2009, Vol. 13, No. 2

Chabrot P, Cardot Jm, Guibert P, Bouculat F, Cassagnes L, Léger-Enreille A, Buc E, Dechelotte P, Bommelaer G, Boyer L, Abergel A Cisplatin Pharmacokinetics In Nontumoral Pig Liver Treated With Intravenous Or Transarterial Hepatic Chemoembolization. Cardiovasc Intervent Radiol. 2012 Apr 24.

Chassaing C, Schmidt J, Eschalier A, Cardot JM, Dubray C. Hyperalgesia Induced By Cutaneous Freeze Injury For Testing Analgesics In Healthy Volunteers. Br. J. Clin. Pharmacol. 61(4) : 389-97, 2006.

Dapoigny M., Piche T., Ducrotte P., Lunaud B., Cardot J-M., Bernalier-Donadille A. Lcr35 Complete Freeze-Dried Culture Versus Placebo In Irritable Bowel Syndrome. World J Gastroenterol 2012 May 7; 18(17): 2067-2075

Déat-Lainé E, Hoffart V, Cardot Jm, Subirade M, Beyssac E. Development And In Vitro Characterization Of Insulin Loaded Whey Protein And Alginate Microparticles. Int J Pharm. 2012 Dec 15;439(1-2):136-44

Déat-Lainé E, Hoffart V, Garrait G, Beyssac E.Whey protein and alginate hydrogel microparticles for insulin intestinal absorption: evaluation of permeability enhancement properties on Caco-2 cells. Int J Pharm. 2013. 10;453(2):336-42.

Déat-Lainé E, Hoffart V, Garrait G, Jarrige Jf, Cardot Jm, Subirade M, Beyssac E Efficacy Of Mucoadhesive Hydrogel Microparticles Of Whey Protein And Alginate For Oral Insulin Delivery, Pharm Res 2012 Oct 24, Pmid: 23064128

Diarrassouba F, Garrait G, Remondetto G, Alvarez P, Beyssac E, Subirade M.Increased stability and protease resistance of the β-lactoglobulin/vitamin D3 complex.Food Chem. 2014. 15;145:646-52.

Dualé C, Dalle N, Cardot Jm, Martin A, Boby H, Bigay V, Dubray C. Validation Of A Transient Pain Monitor In Healthy Volunteers. Reg Anesth Pain Med. 2011 Mar-Apr;36(2):110-5

Dualé C, Guastella V, Morand D, Cardot Jm, Aublet-Cuvelier B, Mulliez A, Schoeffler P, Escande G, Dubray C.Characteristics Of The Neuropathy Induced By Thoracotomy: A 4-Month Follow-Up Study With Psychophysical Examination. Clin J Pain. 2011 Jul-Aug;27(6):471-80

Duale C., Deveau J., Cardot J.M., Grand-Boyer A., Schoeffler P., Dubray C. Cutaneous Amitiptyline In Human Volunteers. Anesthesiology, 2008, 108 : 714-721.

Etienne-Grimaldi M-C, Cardot J-M, François E, Renée N, Douillard J-Y, Gamelin E And Milano G, Chronopharmacokinetics Of Oral Tegafur And Uracil In Colorectal Cancer Patients, Clinical Pharmacology & Therapeutics,2007., 83(3):413-415

Etienne-Grimaldi MC, Francois E, Cardot JM, Renee N, Douillard JY, Gamelin E, Bennouna J, Chateau Y, Milano G. A Clinical Pharmacokinetic Analysis Of Tegafur-Uracil (UFT) Plus Leucovorin Given In A New Twice-Daily Oral Administration Schedule. Clin. Pharmacokinet. 2007, 46(11):953-963.

Etienne-Mesmin L, Livrelli V, Privat M, Denis S, Cardot Jm, Alric M, Blanquet-Diot S. Effect Of A New Probiotic Saccharomyces Cerevisiae Strain On Survival Of Escherichia Coli O157:H7 In A Dynamic Gastrointestinal Model. Appl Environ Microbiol. 2011 Feb;77(3):1127-31. Epub 2010 Dec 3

Etienne-Mesmin L, Livrelli V, Privat M, Denis S, Cardot J-M., Alric M, Blanquet-Diot S., Effect Of A New Probiotic Saccharomyces Cerevisiae Strain On The Survival Of Escherichia Coli O157:H7 In A Dynamic Gastrointestinal Model Appl. Environ. Microbiol. Doi:10.1128/AEM.02130-10

Feria-Gervasio D, Tottey W, Gaci N, Alric M, Cardot Jm, Peyret P, Martin Jf, Pujos E, Sébédio Jl, Brugère Jf. Three-Stage Continuous Culture System With A Self-Generated Anaerobia To Study The Regionalized Metabolism Of The Human Gut Microbiota. J Microbiol Methods. 2013 Dec 11;96c:111-118. Doi: 10.1016

Feria-Gervasio D, Tottey W, Gaci N, Alric M, Cardot Jm, Peyret P, Martin Jf, Pujos E, Sébédio Jl, Brugère Jf. Three-stage continuous culture system with a self-generated anaerobia to study the regionalized metabolism of the human gut microbiota.J Microbiol Methods. 2014, 96, 111-118

Garrait G, Jarrige JF, Blanquet S, Beyssac E, Cardot JM And Alric M. Gastrointestinal Absorption And Urinary Excretion Of Trans-Cinnamic And P-Coumaric Acids In Rats. J. Agric. Food Chem. 2006 , 54, 2944-2950,.

Garrait G., Beyssac E., Subirade M.. Development of a novel drug delivery system: Chitosan nanoparticles entrapped in alginate microparticles. J. Microencap. 2014. Sous presse.

Gauthier P, Cardot J.M, Beyssac E., Aiache Jm. "Single Process For Manufacturing Spheres With A Lipid Base". Pharm. Dev And Tech, 2011, 1-12 Early Online.

Gauthier P. "Le Médicament Pédiatrique, Exemple D’un Travail De Designer Adapté Aux Petits Bobos" Revue Doses, 2008, N°31, 27-31.

Gauthier P. "Santé + Design" Transfert De Connaissance : Les Entretiens De L’ecole De Design De Nantes Atlantique, Cadi, 2008, 29-35, Hors-Série 2008.

Gauthier P. "Spray And Save: A Panorama Of Aerosols In Therapeutics " Aerosol Europe, 2010, Vol 18, N°5, 13-15.

Gauthier P. And Cardot J-M., Developing Drugs For Children And The Adjustment Of Medication—Is It A New Challenge Or An Adaptation Of Past Ideas? J. Pers. Med. 2011, 1, 5-16; Doi:10.3390/Jpm1010005

Gauthier P., "The Ninth Annual European Partnerships And Latest Innovations In Drug Delivery", Therapeutic Delivery, 2011, 2(2), 159-163.

Gauthier P., Cardot J-M., Beyssac E., Aiache J-M. Single Process For Manufacturing Spheres With A Lipid Base (Hco) Pharm Dev Technol () (2010)

Gauthier P., Cardot J-M., Beyssac E., Aiache J-M. Single Process For Manufacturing Spheres With A Lipid Base (Hco) Pharmaceutical Development And Technology, 2011, 1–12.

Gauthier, P.; Cardot, J.-M. Teenagers As A Moving Target: How Can Teenagers Be Encouraged To Accept Treatment? J. Pers. Med. 2012, 2, 277-286.

Gérard-Champod M, Blanquet-Diot S, Cardot J-M, Bravo D, Alric M.,Development And Validation Of A Continuous In Vitro System Reproducing Some Biotic And Abiotic Factors Of The Veal Calf Intestine., Appl Environ Microbiol. 2010 Jun 25. [Epub Ahead Of Print]

Hébrard G, Blanquet S, Beyssac E, Remondetto G, Subirade M And Alric M. Use Of Whey Protein Beads As A New Carrier System For Recombinant Yeasts In Human Digestive Tract. J. Biotechol. 2006 127(1) : 151-160.

Hébrard G, Hoffart V, Beyssac E, Cardot J-M., Alric M, Subirade M. , Coated Whey Protein/Alginate Microparticles As Oral Controlled Delivery Systems For Probiotic Yeast., J Microencapsul, 2010, 27(4): 292-302.

Hébrard G, Hoffart V., Cardot J-M., Subirade M., Beyssac E. Development And Characterization Of Coated-Microparticles Based On Whey Protein/Alginate Using The Encapsulator Device Drug Development And Industrial Pharmacy : 2013; 39(1): 128–137

Hebrard G., Hoffart V., Cardot J-M., Alric M., Subirade M., Beyssac E., Investigation Of Coated Whey Protein/Alginate Beads As Sustained Release Dosage Form In Simulated Gastrointestinal Environment. Drug Dev Ind Pharm, 2009, 35(9): 1103-12.

Hemmingsen P, Haahr A-M., Gunnergaard C., Cardot J-M. Development Of A New Type Of Prolonged Release Hydrocodone Formulation Based On Egalet® Adprem Technology Using In Vivo In Vitro Correlation Pharmaceutics 2011, 3, 73-87; Doi:10.3390/Pharmaceutics3010073

Hemmingsen P., Haahr A-M., Gunnergaard C. And Cardot J-M., Development Of A New Type Of Prolonged Release Hydrocodone Formulation Based On Egalet® Adprem Technology Using In Vivo–In Vitro Correlation, Pharmaceutics 2011, 3, 73-87.

Hoffart V, Lamprecht A, Maincent M, Lecompte T, Vigneron C, Ubrich N. Oral Biovailability Of Low Molecular Weight Heparin Using A Nanoparticulate Polymeric Delivery System. J. Control Release. 2006 113(1) : 38 - 42,

Hoffart V, Maincent M, Lamprecht A, Latger-Cannard V, Regnault V, Merle C, Jouan-Hureaux V, Lecompte T, Vigneron C, Ubrich N. Immunoadsorption Of Alloantibodies Onto Erythroid Membrane Antigens Encapsulated Into Polymeric Microparticles. Pharm. Res. 2007, 24(11): 2055-2062.

Meunier JP, Cardot JM, Gauthier P, Beyssac E, Alric M. Use Of Rotary Fluidized Bed Technology For The Development Of Sustained-Release Plant Extracts Pellets : Potential Application For Feed Additive Delivery. J. Anim. Sci. 2006, 84(7) : 1850-1859.

Meunier JP, Cardot JM, Manzanilla EG, Wysshaar M, Alric M. Use Of Spray-Cooling Technology For Development Of Microencapsulated Capsicum Oleoresin For The Growing Pig As An Alternative To In-Feed Antibiotics: A Study Of Release Using In Vitro Models. J. Anim. Sci. 2007, 85: 2699-2710.

Nosek L, Cardot J-M., Owens D., Ibarra P., Bagate K., Vergnault G., Kaiser K., Fischer A., Heise T, Modified Release Terbutaline (Skp1052) For Hypoglycemia Prevention: A Proof-Of-Concept Study In People With Type 1 Diabetes Mellitus, Diabetes Obes Metab. 2012 Sep 18. Doi: 10.1111/Dom.12003. Pmid: 22988932

Paul C, Zénut M, Dorut A, Coudoré Ma, Vein J, Cardot Jm, Balayssac D..Use of Domperidone as a Galactagogue Drug: A Systematic Review of the Benefit-Risk Ratio. J Hum Lact. 2015 Feb;31(1):57-63.

Paul C, Zénut M, Dorut A, Coudoré Ma, Vein J, Cardot Jm, Balayssac D.. Response to “Use of domperidone to increase breast milk supply: further consideration of the benefit risk ratio is required”J Hum Lact. 2015 in press

Pickering G, Creac’h C, Radat F, Cardot J-M., Alibeu J-P, André G, Chaib S, Douay L, Hirsch F, Malochet V, Navez M, Picard P, Serra E, Sorel M, Simen E, Sergent A, Dubray C, Laurent B. Autogenic Training In Patients Treated For Chronic Headache: A Randomised Clinical Trial J Pain Manage 2012;5(2):

Pickering G, Morel V, Simen E, Cardot Jm, Moustafa F, Delage N, Picard P, Eschalier S, Boulliau S, Dubray C. Oral Magnesium Treatment In Patients With Neuropathic Pain: A Randomized Clinical Trial. Magnes Res. 2011 Jun 1;24(2):28-35.

Pickering G., Moustafa F., Desbrandes S., Cardot J-M., Roux D., Dubray C. Paracetamol And Opioid Pathways:A Pilot Randomized Clinical Trial Fondamental And Clinical Pharmacology, 2013, Doi: 10.1111

Pickering G, Macian N, Libert F, Cardot Jm, Coissard S, Perovitch P, Maury M, Dubray C. Buccal acetaminophen provides fast analgesia: two randomized clinical trials in healthy volunteers. Drug Des Devel Ther. 2014 Sep 26;8:1621-7.

Roudier B, Davit Bm, Beyssac E, Cardot Jm. In Vitro- In Vivo Correlation's Dissolution Limits Setting. Pharm Res. 2014 Sep;31(9):2529-38

Roudier B, Davit B, Schütz H, Cardot Jm. Impact of data base structure in a successful in vitro-in vivo correlation for pharmaceutical products. AAPS J. 2015 Jan;17(1):24-34

Scheubel E, Adamy L, Hoffart V And Cardot J-M. ,Selection Of The Most Suitable Dissolution Method For An Extended Release Formulation Based On Ivivc Level A Obtained On Cynomolgus Monkey, ,Drug Development And Industrial Pharmacy, Nov 2010, Vol. 36, No. 11, Pages 1320-1329: 1320-1329.

Scheubel E., Adamy L. And Cardot J-M., In Vitro Dissolution Of Mycophenolate Mofetil: Comparison Between Innovator And Generic Formulations, Dissolution Technology, 2012, 3, 52-58.

Scheubel E., Lindenberg M., Beyssac E. And Cardot J-M., Small Volume Dissolution Testing As A Powerful Method During Pharmaceutical Development, Pharmaceutics 2010, 2, 351-

Souliman S, Beyssac E, Cardot JM, Denis S, Alric M. Investigation Of The Biopharmaceutical Behaviour Of Theophylline Hydrophilic Matrix Tablets Using USP Methods And An Artificial Digestive System. Drug Dev. Ind. Pharm. 2007, 33(4): 475-483.

Souliman S, Blanquet S, Beyssac E, Cardot JM. A Level A In Vitro / In Vivo Correlation In Fasted And Fed States Using Different Methods: Applied To Solid Immediate Release Oral Dosage Form. Eur. J. Pharm. Sci. 27 : 72-79, 2006.

Xie X, Cardot Jm, Garrait G, Thery V, El-Hajji M, Beyssac E. Micelle dynamic simulation and physicochemical characterization of biorelevant media to reflect gastrointestinal environment in fasted and fed states.Eur J Pharm Biopharm. 2014 Oct;88(2):565-73.

Livres :

Aiache J.M., Beyssac E., Cardot J.M., Hoffart V, Renoux R. Initiation À La Connaissance Du Médicament. Ed. Masson, Abrégés, 2008, 5e Édition

Aiache J.M., Cardot J.M., Hoffart V. Médicaments Et Autres Produits De Santé. Ed. Masson, Pass-Santé, 2011,

Aiache JM, Cardot JM, Beyssac E, Hoffart V. Formes À Liberation Modifiées Destinées Aux Voies Orale Et Parentérale. Dans Collection Le Moniteur Internat Dirigée Par Vaubourdolle M., Tome 4, Médicaments, 3e Édition, 2007, 183-230.

Aiache JM, Cardot JM, Beyssac E., Hoffart V. Les Formes À Libération Modifiée Destinées Aux Voies Orale Et Parentérale. Moniteur De l’Internat, 2006, 6.

Aiache JM, Cardot JM, Beyssac E, Hoffart V. Formes À Liberation Modifiées Destinées Aux Voies Orale Et Parentérale. Dans Collection Le Moniteur Internat Dirigée Par Vaubourdolle M., Tome 4,

Aiache JM, Cardot JM, Beyssac E., Hoffart V. Les Formes À Libération Modifiée Destinées Aux Voies Orale Et Parentérale. Moniteur De l’Internat, 2013, Tome 6.

Beyssac E., Billon A., Gauthier H., Skiba M., Vandamme Th., Gélules Capsules Molles Et Contrôle Biopharmaceutique Des Formes Orale Solides, In Pharmacie Galénique, 2008, Masson.

Cardot JM and Beyssac E. In Vivo In Vitro Correlation. Fourth Edition Of The Encyclopedia Of Pharmaceutical Technology. Ed. James Swarbrick, James C. Boylan, Informa. Inc. New York, London.: 2011.

Cardot JM and Beyssac E. In Vivo In Vitro Correlation. Third Edition Of The Encyclopedia Of Pharmaceutical Technology. Ed. James Swarbrick, James C. Boylan, Marcel Dekker. Inc. New York, Basel, Hong Kong. Vol 1.: 2006, 2062-2072.

Cardot JM, Piccerelle Ph. Et Wehrlé P. Outils Statistiques Pour L’optimisation Et Le Suivi De La Qualité Des Formes Et Des Procédés Pharmaceutiques. In Pharmacie Galénique, Ouvrage Collectif Sous La Direction De P. Wherlé, 2008, 321-355.

Cardot J-M. Beyssac E. Cleaning Vs Dedicated Or Disposable Equipment For Pharmaceutical Production, In Advances In Large Scale Bio-Manufacturing And Scale Up Production, ASM Press And Institute Of Science And Technology Management, Washington, 3rd Ed, 2007, 767-776.

Cardot J-M.,Applying IVIVC To Biodrug Development, In Biodrug Delivery Systems Fundamentals, Applications And Clinical Development, Mariko Morishita And Kinam Park Editors, Informa Press 2010, 341-348

Aiache J-M. , Cardot J-M., Hoffart V, Médicaments et autres produits de santé Elsevier-Masson, 2011

Cardot J-M. Beyssac E. Chapter 174 In vitro-In vivo Correlations In Encyclopaedia of Pharmaceutical sciences, 4th Ed Editor, Taylor and Francis Group. 2012 Volume 1 No. 1








dernière mise à jour le 18/02/2015 par Jean-Michel CARDOT

Clermont Université Université Clermont Auvergne